Sticky-end or blunt end DNA ligation in only 5 Min at room temperature.
Inno 5 Min DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 Min at room temperature. The kit contains T4 DNA Ligase and a specially formulated 5C Rapid Ligation buffer optimized for fast and drricent DNA ligation.
The efficiency of this fast ligation is analogous to that obtained with T4 DNA Ligase in a standard 1 hour ligation. The ligation reaction mixure can be used directly for bacterial transformation.
Ligation of sticky-end and blunt-end DNA fragments with Inno 5 Min DAN Ligation Kit. Blunt-end ligation. pUC19 DNA/SmaI dephosphorylated vector was ligated with 1 DAN/PvuII fragment(2.3kb) Sticky-end ligation, pUC19 DNA/PstI dephosphorylated vector was ligated with 1 DAN/PvuI fragment(2.1kb) The efficiency of ligation was estimated by transformation of E.coli XL1-Blue cells.
Ordering Information
PRODUCT NAME
NO.
SIZE
Inno 5 Min DNA Ligation Kit
T4 DNA Ligase
T4 DNA Ligase
Ligation of sticky-end and blunt-end DNA fragments with Inno 5 Min DAN Ligation Kit. 
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