InnoSure PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. InnoSure PCR Master Mix provides superior yields in both routine and challenging PCR reduces smearing and virtually eliminates unwanted background artifacts.
InnoSure PCR Master Mix contains red and yellow loading dyes to allow loading of PCR product directly on gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample. The red dye runs in a range between 500bp(2% gles) and 1500bp(0.8% gels) and the yellow dye runs at less than 10bp. InnoSure PCR Master Mix contains a fixed MgCl2 concentration of 1.75mM. However, higher concentrations may be achived by adding additional MgCl2.
Target 3kb was generated from 20ng various
genomic DNAs using InnoSure Master Mix.
All extensions were 1min. per kb.
Each reaction was assembled at room temperature.
Easy-to-See, Easy-to-Load
Load directly to gel from
thermocycler No additional
loading buffer needed Separates into 2 colors
during gel electrophoresis
Convenient red and yellow dye format means no need to add loading dye Higher specificity fidelity, and performance Greater DNA amplification 2X PCR Master Buffer inclucing Mg++ and dNTPs