InnoXpand PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. InnoXpand PCR Master Mix is composed of a unique enzyme blend containing two thermostable polymerases : Taq DNA polymerase and a thermostable polymerase with proofreading activity. InnoXpand PCR Master Mix is designed to amplify fragments up to 5kb with high yield and fidelity, as well as with high specificity form esisomal and genomic DNA.
InnoXpand PCR Master Mix is contains red and yellow loading dyes to allow loading of PCR product directly on a gel after thermal cycling, minimizing pipetting wteps and providing ease visualization of sample.
The red dye runs in a rage between 500bp(2% gels) and 1500bp(0.8% gels) and the yellow dye runs at less than 10bp. InnoXpand PCR Master Mix contains a fixed MgCl2 concentration of 1.75mM. However, higher concentrations may be achieved by adding additional MgCl2
Figure2
Varying am ounts of K562 human genomic
DNA were amplified using InnoXpand PCR
Master mix. Each reaction was
assembled at room temperature.
Benefits
Consistent Results
Lot-to-Lot
Produce consistent resuls : tube-to-tube, experiment-to-experiment Expect the same result with every run because of Lot-to-Lot consistency
Figure2
Produce consistent resuls : tube-to-tube, experiment-to-experiment
